Biology 325: Lecture 8- Functional Genomics

Outline;

I. Functional Genomics

II. RNA Expression Analysis: the Transcriptome

III. Proteomics

IV. Using Genomics to find and study Mendelian disease genes

I. Functional Genomics

II. RNA Expression Analysis: the Transcriptome

A. Microarrays

1. Basics of Microarrays

2. Types of Microarrays

i. Mechanical Spotting: “Spotted Microarrays”

a. Production

b. Analysis

ii. Photolithography: Affymetrix microarrays = “GeneChips”

a. Production

b. Analysis

III. Proteomics

A. Techniques

i. 2-D gel electrophoresis

ii. Mass Spectrometry

B. Example

IV. Using Genomics to find and study Mendelian disease genes

A. Homology Searches

B. Positional Cloning/Chromosome Walking

C. Knockout Organisms